Immunohistochemistry

Immunohistochemistry (IHC) it involves the process of selectively identifying antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC takes its name from the roots "immuno", which simply means antibodies used in the procedure, and "histo", means. Immunohistochemistry was been conceptualized by Albert Coons. 
Sample Preparation.
This involve proper tissue collection, fixation and sectioning. A solution of formalin is often used to fix tissue, but other methods may also be used.