from Mesolithic and Neolithic Ukraine (9500-6000 BC). These were classified as intermediate between EHG and SHG. The males belonged exclusively to R haplotypes (particularly subclades of R1b1 and R1a) and I haplotypes (particularly subclades of I2). Mitochondrial DNA belonged almost exclusively to U (particularly subclades of U5 and U4). A large number of individuals from the Zvejnieki burial ground, which mostly belonged to the Kunda culture and Narva culture in the eastern Baltic, were analyzed. These individuals were mostly of WHG descent in the earlier phases, but over time EHG ancestry became predominant. The Y-DNA of this site belonged almost exclusively to haplotypes of haplogroup R1b1a1a and I2a1. The mtDNA belonged exclusively to haplogroup U (particularly subclades of U2, U4 and U5). Forty individuals from three sites of the Iron Gates Mesolithic in the Balkans were estimated to be of 85% WHG and 15% EHG descent. The males at these sites carried exclusively R1b1a and I (mostly subclades of I2a) haplotypes. mtDNA belonged mostly to U (particularly subclades of U5 and U4). People of the Cucuteni–Trypillia culture were found to harbor about 20% hunter-gatherer ancestry, which was intermediate between EHG and WHG. Narasimshan et al. (2019) coined a new ancestral component, West Siberian Hunter-Gatherer (WSHG). WSHGs contained about 30% EHG ancestry, 50% ANE ancestry, and 20% East Asian ancestry. Unlike the Yamnaya culture, in the Dnieper–Donets culture no Caucasian Hunter-Gatherer (CHG) or Early European Farmer (EEF) ancestry has been detected. Dnieper-Donets males and Yamnaya males carry the same paternal haplogroups (R1b and I2a), suggesting that the CHG and EEF admixture among the Yamnaya came through EHG males mixing with EEF and CHG females. According to David W. Anthony, this suggests that the Indo-European languages were initially spoken by EHGs living in Eastern Europe. Physical appearance The EHGs are suggested to have had mostly brown eyes and light skin, with "intermediate frequencies of the blue-eye variants" and "high frequencies of the light-skin variants." An EHG from Karelia was determined by to have high probabilities of being brown-eyed and dark haired, with a predicted intermediate skin tone. Another EHG from Samara was predicted to be light skinned, and was determined to have a high probability of being blue-eyed with a light hair shade, with a 75% calculated probability of being blond-haired. The rs12821256 allele of the KITLG gene that controls melanocyte development and melanin synthesis, which is associated with blond hair and