{"issue":{"pull_request":{"diff_url":null,"html_url":null,"patch_url":null},"state":"open","user":{"avatar_url":"https://secure.gravatar.com/avatar/840a3abaf67e9fef8c58225d2e20608a?d=https://a248.e.akamai.net/assets.github.com%2Fimages%2Fgravatars%2Fgravatar-140.png","gravatar_id":"840a3abaf67e9fef8c58225d2e20608a","url":"https://api.github.com/users/brainstorm","login":"brainstorm","id":175587},"milestone":null,"labels":[{"name":"asap","color":"02d7e1","url":"https://api.github.com/repos/brainstorm/bcbb/labels/asap"},{"name":"feature","color":"3809f2","url":"https://api.github.com/repos/brainstorm/bcbb/labels/feature"}],"assignee":{"avatar_url":"https://secure.gravatar.com/avatar/8096c7d6bc0d0ee0e67538d947869b3a?d=https://a248.e.akamai.net/assets.github.com%2Fimages%2Fgravatars%2Fgravatar-140.png","gravatar_id":"8096c7d6bc0d0ee0e67538d947869b3a","url":"https://api.github.com/users/vals","login":"vals","id":668803},"updated_at":"2011-10-28T13:10:13Z","closed_at":null,"comments":9,"url":"https://api.github.com/repos/brainstorm/bcbb/issues/20","body":"Instead of blindly trusting the samplesheet/manually specified barcodes, Clustering them, in order to find contamination or other problems.\r\n\r\nIn other words, we should be able to detect which barcodes are there just by processing the fastq files (since we know that the barcodes are attached at the 3' end by default).\r\n\r\n\r\nSuggested by Ellen, Max and others.","html_url":"https://github.com/brainstorm/bcbb/issues/20","title":"Demultiplex based on barcodes found in reads","number":20,"id":1074028,"created_at":"2011-06-17T15:02:31Z"},"action":"created","comment":{"user":{"avatar_url":"https://secure.gravatar.com/avatar/8096c7d6bc0d0ee0e67538d947869b3a?d=https://a248.e.akamai.net/assets.github.com%2Fimages%2Fgravatars%2Fgravatar-140.png","gravatar_id":"8096c7d6bc0d0ee0e67538d947869b3a","url":"https://api.github.com/users/vals","login":"vals","id":668803},"updated_at":"2011-10-28T13:10:13Z","url":"https://api.github.com/repos/brainstorm/bcbb/issues/comments/2555817","body":"Sure, I'll put it as a constant straight in bcbio.solexa","id":2555817,"created_at":"2011-10-28T13:10:13Z"}}