src/toil_lib/tools/__init__.py
def get_mean_insert_size(work_dir, bam_name):
    """Function taken from MC3 Pipeline"""
    cmd = "docker run --log-driver=none --rm -v {}:/data quay.io/ucsc_cgl/samtools " \
          "view -f66 {}".format(work_dir, os.path.join(work_dir, bam_name))
    process = subprocess.Popen(args=cmd, shell=True, stdout=subprocess.PIPE)
    b_sum = 0.0
    b_count = 0.0
    while True:
        line = process.stdout.readline()
        if not line:
            break
        tmp = line.split("\t")
        if abs(long(tmp[8])) < 10000:
            b_sum += abs(long(tmp[8]))
            b_count += 1
    process.wait()
    try:
        mean = b_sum / b_count
    except ZeroDivisionError:
        mean = 150
    print "Using insert size: %d" % mean
    return int(mean)
def get_mean_insert_size(work_dir, bam_name):
    """Function taken from MC3 Pipeline"""
    cmd = "docker run --log-driver=none --rm -v {}:/data quay.io/ucsc_cgl/samtools " \
          "view -f66 {}".format(work_dir, os.path.join(work_dir, bam_name))
    process = subprocess.Popen(args=cmd, shell=True, stdout=subprocess.PIPE)
    b_sum = 0.0
    b_count = 0.0
    while True:
        line = process.stdout.readline()
        if not line:
            break
        tmp = line.split("\t")
        if abs(long(tmp[8])) < 10000:
            b_sum += abs(long(tmp[8]))
            b_count += 1
    process.wait()
    try:
        mean = b_sum / b_count
    except ZeroDivisionError:
        mean = 150
    print "Using insert size: %d" % mean
    return int(mean)
Function taken from MC3 Pipeline