Figure 1. Experimental design. Male A/J mice were given 6 weekly intraperitoneal injections of AOM (10 mg/kg; n = 40) or 0.9% saline (n = 35) beginning at 5 weeks of age. Feces were collected 3, 5, and 7 weeks after the last injection; plasma was collected 5 and 7 weeks after the last injection; tumor tissue from AOM-injected mice and colorectal mucosa from saline-injected mice were harvested 7 weeks after the last injection. Separate groups of mice (n = 5 mice/time point) were used to determine tumor burden and histology 3, 5, and 7 weeks following the last AOM injection.