RhizoFlux platform for phenotyping of multiple ion uptake kinetics in plants. (A) Maize seeds are surface sterilized and germinated on germination paper rolls. (B) Evenly germinated seedlings are transferred to (C) seedling cones and grown in (D) aerated hydroponics for 14 days. (E) Plants are transferred to a custom ion uptake setup consisting of 24 hydroponic chambers connected to two <t>24-channel</t> peristaltic pumps for simultaneous solution loading, aeration, and sampling onto a collection plate. (F) Ion concentrations of collected nutrient samples are determined using ion chromatography (G) for quantifying ion depletion across time and (H) used to calculate net specific ion uptake rates on a root length or mass basis. 