NCSTN / Nicastrin Antibody - Anti-NCSTN rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 78 kDa. Observed band size: 130 kDa.