9 Immunoassay Calibration CASE#1: CASE#1: C1 & C2 within calculation threshold the master curve is left as it is CASE#2: CASE#2: C1 & C2 within the acceptance threshold the master curve is accepted and adjusted Checking the Curve CASE#3: CASE#3: C1 & C2 are outside the acceptance threshold Invalid curve troubleshoot and repeat controls OR perform 6 points standardization