Predicted band size: 76 kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&amp;L (HRP) at 1/10000 dilution Exposure time: 2 minutes)