Fig. 2. Green fluorescent protein (GFP) reveals the cellular morphology and integrity of dentate granule cells in control and anesthesia-exposed mice. Images show representative cells after a 6-h exposure to room air or isoflurane. Isoflurane dramatically increased caspase-3 immunoreactivity. Arrows denote double-labeled cells. In the anesthesia-treated animal, the two cells in the center of the image have short, aspiny dendrites projecting into the dentate molecular layer; morphologic features of immature granule cells. Morphology suggests cellular disintegration. Scale bar = 20 μm.