The process of cell isolation from the epidermis (first A to end J). 1, The subcutaneous tissue and dermis were removed; 2, the remaining skin was cut into small sections (0.5 mm thick); 3, they were placed in 0.25% neutral Dispase overnight at 4°C to obtain the epidermis; 4, 5, and 6, dermis was removed from epidermis; 6 and 7, immersed in a solution trypsin/EDTA at 37°C for 5 min; this digestion was terminated by the addition of the serum; 8, single cell suspensions were obtained by pipette blowing, filtered through a 200 mesh filter for screening and centrifuged twice at 1,500 rpm for 6 min; 9, cell morphology was observed using inverted microscope.