Step 3: Cranking up the Band (BioPharmaceuticals) Assembling a team of scientists to sort through the different molecules to find the specific one with the right mixture of properties –Affinity for Target ELISA, HTRF, SPR (Biacore), BLI (Octet RED) –Ability and potency to inhibit desired biology Cell or plasma based signaling assays –Pharmacokinetics/ Pharmacodynamics (PK/PD) –Other Biophysical Properties Expression yield (system dependent) Melting temperature and behavior –Low temperature, irreversible melting behavior very undesirable Aggregate formation on storage Properties can be tweaked by addition of point mutations at this step