Figure 1. Time schema for protocol. The warm group was treated similarly to the cold groups except that the warm group did not undergo 22 h of hypothermia or exposure to isoflurane or glibenclamide (GLIB). C1 and C2 = initial and final controls; D1 and D2 = initial and final responses to adenosine (ADE), 5 hydroxytryptamine (5-HT), nitroprusside (NP), and epinephrine (EPI). The five cold groups are: C = cold control; 1 (1.3%) and 2 (2.6%), isoflurane alone or with GLIB.