Identification of the recombinant plasmid <t>pNZ8149-SECF1S1</t> by PCR and restriction enzyme analysis (a) PCR amplification of F1S1gene. Lane A: DNA ladder; Lanes B and C: PCR products of the F1S1 gene amplification D: No template control. (b) Restriction analysis of the recombinant plasmid pNZ8149-SECF1S1. Lane A: DNA ladder; Lane B: pNZ8149-SECF1S1 double-digested with <t>Kpn</t> I and Xba I; C: pNZ8149-SECF1S1 digested with Kpn I. 