Immunocytochemistry analysis of LOVO cells labeling Emi1 with Rabbit anti-Emi1 antibody (ET7108-20) at 1/50 dilution.<br />
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Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Emi1 antibody (ET7108-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG  was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.