Predicted band size: 47 kDa . Positive control: 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&amp;L (HRP) at 1/10000 dilution Exposure time: 1 minute)