Figure 1. Constitutive tyrosine-phosphorylation of FGFR3 in KMS-11 cells lysed in the presence of phosphatase inhibitors (+) is detected by PathScan<sup>®</sup> Phospho-FGF Receptor 3 (panTyr) Chemiluminescent Sandwich ELISA Kit #64740. In contrast, a low level of phospho-FGFR3 protein is detected in KMS-11 cells lysed in the absence of phosphatase inhibitors* (-). For the FGFR3-negative RPMI 8226 cells, there is no phospho-FGFR3 detected by this ELISA kit in either presence or absence of phosphatase inhibitors. Immediate light generation with chemiluminescent substrate is shown. *Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate, and sodium orthovanadate.