Figure 6. Representative images of the reconstructed DG of a mouse 2 months after irradiation with 0 Gy (A) or 10 Gy (B). Nuclei were counterstained (green); activated microglia (CD68+; red). A typical activated microglial cell (inset). Irradiation is associated with increased numbers of activated microglia. Irradiated animals exhibited a significant increase in the average number of activated microglia cell/mm2 (C). Points, mean averages of six mice; bars, SE (*, P < 0.05). Bar, 100 μm.