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Baseball field
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The baseball diamond of the San Diego Padres' Petco Park, seen from the left-field stands.
A baseball field, also called a ball field, sandlot or a baseball diamond, is the field upon which the game of baseball is played. The term can also be used as a metonym for a baseball park.
Contents
[hide]
1 Specifications
2 First base
3 Second base
4 Third base
5 Home plate
6 Batter's box and catcher's box
7 Foul poles
8 Pitcher's mound
9 Baseline
9.1 Running baseline
9.2 Running lane
10 Grass line
11 Outfield
12 Warning track
13 Outfield wall
14 Bullpen
15 On-deck circles
16 Coach's boxes
17 History
18 Maintenance
19 Honors and awards
20 See also
21 References
22 External links
Specifications[edit]
This section appears to contradict itself on what "the infield" means. Please see the talk page for more information. (January 2017)
Unless otherwise noted, the specifications discussed in this section refer to those described within the Official Baseball Rules, under which Major League Baseball is played.[1]
Diagram of a baseball field.
The starting point for much of the action on the field is home plate, which is a five-sided slab of whitened rubber, 17 inches (43.2 cm) square with two of the corners removed so that one edge is 17 inches (43.2 cm) long, two adjacent sides are 8.5 inches (21.6 cm) and the remaining two sides are 12 inches (30.5 cm) and set at an angle to make a point. Adjacent to each of the two parallel 8.5-inch (21.6 cm) sides is a batter's box. The point of home plate where the two 12-inch (30.5 cm) sides meet at right angles is at one corner of a ninety-foot (27.5 m) square. The other three corners of the square, in counterclockwise order from home plate, are called first base, second base, and third base. Three canvas bags fifteen inches (38 cm) square mark the three bases. These three bags along with home plate form the four points at the corners of the infield.
All the bases, including home plate, lie entirely within fair territory. Thus, any batted ball that touches those bases must necessarily be in fair territory. While the first and third base bags are placed so that they lie inside the 90-foot (27.5 m) square formed by the bases, the second base bag is placed so that its center (unlike first, third and home) coincides exactly with the "point" of the ninety-foot (27.5 m) infield square. Thus, although the "points" of the bases are 90 feet (27.5 m) apart, the physical distance between each successive pair of base markers is closer to 88 feet (26.8 m).[2]
The lines from home plate to first and third bases extend to the nearest fence, stand or other obstruction and are called the foul lines. The portion of the playing field between (and including) the foul lines is fair territory; the rest is "foul territory." The area in the vicinity of the square formed by the bases is called the infield; fair territory outside the infield is known as the outfield. Most baseball fields are enclosed with a fence that marks the outer edge of the outfield. The fence is usually set at a distance ranging from 300 to 420 feet (91 to 128 m) from home plate. Most professional and college baseball fields have a right and left foul pole. These poles are at the intersection of the foul lines and the respective ends of the outfield fence and, unless otherwise specified within the ground rules, lie in fair territory. Thus, a batted ball that passes over the outfield wall in flight and touches the foul pole is a fair ball and the batter is awarded a home run.
First base[edit]
Main article: First baseman
Left field foul pole at Citi Field in Flushing, Queens.
First base is the first of four bases that must be touched by a player on the batting team in order to score a run. Unlike when an offensive player reaches second or third base, it is permissible for a batter-runner to overrun first base without being in jeopardy of being put out. After contact is made with the base, the batter-runner may slow down and return to first base at his leisure, so long as he makes no move or attempt to advance to second base. The runner cannot be tagged out if he is touching the base with any part of his body.
The first baseman is the defensive player responsible for the area near first base. A professional first baseman is often a slow runner and tall.[citation needed] A tall first baseman presents a large target to which other fielders can throw, and his height gives him a larger range in reaching and catching errant throws. Players who are left-handed are marginally preferable for first base because: first, it is easier for a left-handed fielder to catch a pick-off throw from the pitcher and tag the baserunner; and, second, it is easier for a left handed thrower to make the throw to second base to start a 3-6-3, 3-6-4, or a 3-6-1 double play.[citation needed] Also, a right-handed first baseman must, when setting himself up to receive a throw from an infielder, execute a half-pivot near the base; this is a move that a left-hander need not make (this advantage is very marginal, however). There are three infield positions that can only effectively be occupied by right-handed players: 2nd base, 3rd base, and shortstop. This is because of the time it takes to pivot and set to throw. It takes a left-handed thrower more time to make that pivot and in the fast-paced major league game, that time is critical. As a result, there are fewer positions a left-handed player can occupy, and if that player is not fast, the outfield may not be a good fit.
In the numbering system used to record defensive plays, the first baseman is assigned the number '3'.
Second base[edit]
Main article: Second baseman
Second base is the second of four stations on a baseball diamond which must be touched in succession by a base runner in order to score a run for that player's team. Second base is typically defended by the second baseman and the shortstop. Second base is also known as the keystone sack. A runner on second base is said to be in "scoring position", owing to the high likelihood of reaching home plate and scoring a run from second base on most base hits. Since second is the farthest base from home plate, it is the most common target of base stealing. The runner cannot be tagged out if he is touching the base with any part of his body.
Ideally, the second baseman and shortstop possess quick hands and feet and the ability to release the ball rapidly and with accuracy. One will usually cover second base when the other attempts to field the ball. Both players must communicate well to be able to make a double play. Particular agility is required of the second baseman in double play situations, which usually forces the player to throw towards first while his momentum carries him in the opposite direction. In the numbering system used to record defensive plays, the second baseman is assigned the number 4, and the shortstop 6.
Third base[edit]
Andy Wilkins rounds third base following a home run for the 2010 Arkansas Razorbacks baseball team.
Main article: Third baseman
Third base is the third of four bases a base runner must touch in a counterclockwise succession in order to score a run. Many batted balls that result in the batter being put out (such as a sacrifice fly) may nevertheless allow a runner to reach home plate and score a run from third base, provided that the third and final out is not recorded before he can do so. A runner on third base is therefore particularly valuable to the batting team when fewer than two outs have been recorded. The runner cannot be tagged out if he is touching the base with any part of his body.
The third baseman is the defensive player whose responsibility is to defend the area nearest to third base. A third baseman ideally possesses quick reaction to batted balls and a strong arm to make the long throw to first base. The distance from 3rd to 1st is about 127 feet. In the numbering system used to record defensive plays, the third baseman is assigned the number 5.
Home plate[edit]
"Home plate" redirects here. For other uses, see Home plate (disambiguation).
Home plate of a baseball field
Home plate, formally designated home base in the rules, is the final base that a player must touch to score. Unlike the other bases, home plate is a five-sided slab of whitened rubber that is set at ground level. The use of rubber was developed by Robert Keating, who pitched one game for the 1887 Baltimore Orioles. Previously—and more dangerously—the plate was made of stone, iron, or wood.[3]
The dimensions of home plate are 17 inches (43.2 cm) across the front, 8.5 inches (21.6 cm) down each side, with a triangular rear of two sides of 12 inches (30.5 cm). The rear edges are at 45 degrees to the sides, making a point at the back.[1] The plate is white and surrounded by a black strip 3/4 inches (1.9 cm) in width. The plate sits entirely in "fair" territory, with the two rear edges aligned with the right and left field foul lines. The length and angle requirements for home plate mandate that it is not a regular pentagon; it is a 17 inches by 8.5 inches (43.2 x 21.6 cm) rectangle with an isosceles triangle of base 17 inches (43.2 cm) and equal sides of 12 inches (30.5 cm) attached to the back.
Both the Major League Baseball Official Rules and the Little League rulebook say the angle between the two 12 inch sides should be 90 degrees. However, the hypotenuse of a right angled triangle with two sides of 12 inches is 288 {\displaystyle {\sqrt {288}}} inches, which is slightly less than the 17 inches required. The home plate as specified is therefore not physically realizable.[4]
50 to 100 feet (15 to 30 meters) behind home plate is the backstop, which is a wall/fence that will stop wild pitches, passed balls, and foul balls. In enclosed stadiums, the backstop is often composed of a lower part, which is like any other part of the wall, and an upper netting to protect spectators seated behind it; in recreational baseball fields, there is usually a tall chain-link fence, including an angled top section, composing the entire backstop.
Batter's box and catcher's box[edit]
David Ortiz (in gray) of the Boston Red Sox stands in the left-handed hitters' batter's box at U.S. Cellular Field against the Chicago White Sox on July 7, 2006.
The batter's box is the place where the batter stands when ready to receive a pitch from the pitcher. It is usually drawn in chalk on the dirt surrounding home plate, and the insides of the boxes are watered down before each game.
The chalk lines delineating the two Foul Lines are rarely extended through the batter's boxes. However, those lines exist conceptually for the purpose of judging a batted ball fair or foul. In addition, inside edges of the Batter's Boxes are often not laid-in with chalk. Similarly, though not marked, those lines continue to exist for the purpose of the rules pertaining to the batter's box and the batter's position relative thereto.
There are two batter's boxes, one on each side of home plate. The batter's boxes are 4 feet wide (1.2 m) and 6 feet long (1.8 m). The batter's boxes are centered lengthwise at the center of home plate with the inside line of each batter's box 6 inches (15.24 cm) from the near edge of home plate. A right-handed batter would stand in the batter's box on the left side of home plate from the perspective of the catcher and umpire. A left-handed batter would stand in the batter's box to their right. A batter may only occupy one batter's box at a time and may not legally leave the batter's box after the pitcher has come set or has started his windup. Should the batter wish to leave the batter's box once the pitcher has engaged the rubber, he must first ask the umpire for time-out. Time will not be granted if the pitcher has already started his pitching motion. For playing rules relating to the batter's box, see Rules 6.05 and 6.06 of the Official Baseball Rules.
Foul poles[edit]
One of two foul poles at Citizens Bank Park, Philadelphia.
Foul poles, if present, help umpires judge whether a fly ball hit above the fence line is foul (out of play) or fair (a home run). The poles are a vertical extension of the foul lines at the edge of the field of play. The outer edge of the foul lines and foul poles define foul territory. Both the lines and the poles are in fair territory, in contrast to football and basketball, where the lines marking the playing boundaries are out of bounds. The minimum distance to hit a home run (along either foul line) is set by baseball rules, generally at 325 feet (99 m).[5]
Before 1920,[citation needed] the foul lines extended indefinitely; a batter was awarded a home run only if a fly ball out of the field was fair where it landed, or "when last seen" by the umpire. Now, a batted ball that leaves the field in flight is judged fair or foul at the point it leaves the field. Thus, such a fly ball passing on the fair side of a foul pole, or hitting a foul pole, is a home run regardless of where the ball goes thereafter.
Foul poles are typically much higher than the top of the outfield fence or wall, and often have a narrow screen running along the fair side of the pole. This further aids the umpires' judgment, as a ball that bounces off this screen is a home run. It can still be a difficult call, especially in ballparks with no outfield stands behind the poles to provide perspective. Wrigley Field is notorious for arguments over long, curving flies down a foul line (most notably in left field) that sail higher than the foul pole.
At Major League Baseball fields, foul poles are usually yellow. Those at Citi Field are orange. At the Rogers Centre, there are no foul poles, but large nets suspended from the roof that serve the same purpose. At Petco Park, there is no foul pole in left field; the pole's function is served by a yellow metal strip along the corner of the Western Metal Supply Co. building.
Pitcher's mound[edit]
The pitcher moves forward off the rubber as the pitch is released.
In roughly the middle of the square, equidistant between first and third base, and a few feet closer to home plate than to second base, is a low artificial hill called the pitcher's mound. This is where the pitcher stands when throwing the pitch. Atop the mound is a white rubber slab, called the pitcher's plate or pitcher's rubber. It measures 6 inches (15 cm) front-to-back and 2 feet (61 cm) across, the front of which is exactly 60 feet 6 inches (18.44 m) from the rear point of home plate. This peculiar distance was set by the rule makers in 1893, not due to a clerical or surveying error as popular myth has it, but intentionally (further details in History section).
In Major League Baseball, a regulation mound is 18 feet (5.5 m) in diameter, with the center 59 feet (18 m) from the rear point of home plate, on the line between home plate and second base. The front edge of the pitcher's plate or rubber is 18 inches (46 cm) behind the center of the mound, making the front edge's midpoint 60 feet 6 inches (18.44 m) from the rear point of home plate. Six inches (15.2 cm) in front of the pitcher's rubber the mound begins to slope downward. The top of the rubber is to be no higher than ten inches (25.4 cm) above home plate. From 1903 through 1968, this height limit was set at 15 inches (38.1 cm), but was often slightly higher, sometimes as high as 20 inches (51 cm), especially for teams that emphasized pitching, such as the Los Angeles Dodgers, who were reputed to have the highest mound in the majors.
A pitcher will push off the rubber with his foot in order to gain velocity toward home plate when pitching. In addition, a higher mound generally favors the pitcher. With the height advantage, the pitcher gains more leverage and can put more downward velocity on the ball, making it more difficult for the batter to strike the ball squarely with the bat. The lowering of the mound in 1969 was intended to "increase the batting" once again, as pitching had become increasingly dominant, reaching its peak the prior year; 1968 is known among baseball historians as "The Year of the Pitcher". This restrictive rule apparently did its job, contributing to the hitting surge of modern baseball.
A pitcher's mound is difficult for groundskeepers to maintain. Usually before every game it is watered down to keep the dust from spreading. On youth and amateur baseball fields, the mound may be much different from the rule book definition due to erosion and repair attempts. Even in the major leagues, each mound gains its own character, as pitchers are allowed to kick away pieces of dirt in their way, thereby sculpting the mound a bit to their preference.
The pitcher may keep a rosin bag on the rear of the mound to dry off his hands. Major League Baseball teams are also permitted cleat cleaners on the back of the mound. This may be a flat grate-style plate, or simply a hand tool such as a piece of wood used to remove mud and dirt from cleats. These items are allowed to remain on the backside of the mound at the discretion of the umpire, thus reducing the probability that they will affect a live play.[6]
Baseline[edit]
A baseline is the straight line between two adjacent bases. Baselines are not drawn on the field, although the foul lines serve to mark the baseline between home plate and first base, and between third base and home.
Running baseline[edit]
Generally, base runners are not required to follow the baseline. A base runner seeking to advance more than one base typically "rounds" the base, following a more circular path. However, a base runner's left-right motion is constrained when the defense tries to tag him. At the moment the defense begins the attempt, the baserunner's running baseline is established as a direct line from his current position to the base he is trying for. A runner straying more than three feet (1 m) away from this baseline to avoid a tag may be called out.
Running lane[edit]
Beginning halfway between home and first base, and ending at first base, there is a second chalk line to the right of the foul line. This second line and the part of the foul line it runs parallel to, form the running lane that defines the path in which a batter-runner must run as he is advancing to first base. Rule 6.05(k) of the Official Baseball Rules states that, if a batter-runner running to first base runs outside the running lane, and "in doing so" interferes with the fielder taking the throw at first, then the batter-runner is automatically out. First base itself is not located in the running lane, but Rule 6.05 lets the batter-runner leave the running lane "by means of a step, stride or slide in the immediate vicinity of first base" to step on first base.[7]
Grass line[edit]
The H.H.H. Metrodome, showing a white "grass" line.
The grass line, where the dirt of the infield ends and the grass of the outfield begins, has no special significance to the rules of the game, but it can influence the outcome of a game. Dirt running paths between the bases (and, at one time and still in some parks, between the pitcher and the catcher) have existed since the beginning of the game, although they were not mentioned in the rule books until around 1950, and their specifications are flexible. In addition to providing a running path, the grass lines act as a visual aid so that players, umpires and fans may better judge distance from the center of the diamond. Occasionally the ball may take a tricky bounce off the dirt area or the edge between the dirt and the grass. Multiple World Series championships (including 1924, 1960 and 1986) have been decided or heavily influenced by erratic hops of ground balls.
In artificial turf stadiums, infield dirt was originally only placed around the bases and around the pitcher's and batting areas, which are referred to as "sliding pits." In this configuration, the "grass line" is usually designated with a white arc. Among Major League Baseball fields, Rogers Centre was the last stadium to maintain this type of configuration and is reconfigured with a full dirt infield starting in the 2016 MLB season.[8]
In some college baseball parks with artificial turf fields, the entire field (along with possibly the pitcher's mound) is made up of turf, with parts of the field mainly containing dirt instead merely being clay-colored turf.
Outfield[edit]
The outfield is made from thick grass or artificial turf. It is where the outfielders play. The positions to play in the outfield are right, center, and left field (named in relation to the batter's position; thus left field is beyond third base and right field is beyond first base). Outfields vary in size and shape depending on the overall size and shape of the playing field. The outfield stretches from the infield to the outfield wall and it contains the warning track. Outfields especially vary from Little League to Major League fields. Little League outfields vary more in size than Major League outfields. Outfields often differ from infields in the specific type of grass used, but most Major League outfields are grass.[9]
Warning track[edit]
The bottom of the picture shows part of the orange-colored clay warning track at Fenway Park.
The warning track is the strip of dirt at the edges of the baseball field (especially in front of the home run fence and along the left and right sides of a field). Because the warning track's color and feel differ from the grass field, a fielder can remain focused on a fly ball near the fence and measure his proximity to the fence while attempting to catch the ball safely.
A warning track's width is not specified in the rules. It is generally designed to give about three steps of warning to the highest-level players using the field. Typical widths run from about six feet for Little League fields to about 10–15 feet (3 - 4.5 m) for college- or professional-level play. A warning track this wide also lets groundskeepers avoid driving maintenance vehicles on the grass.
The track can be composed of finely ground rock particles such as cinders, which is why announcer Bob Wolff called it the "cinder path" rather than the "warning track".
The idea of a warning track originated in Yankee Stadium, where an actual running track was built for use in track and field events. When ballpark designers saw how the track helped fielders, it soon became a feature of every ballpark.
Single-minded fielders often crash into a wall trying to make a catch despite the warning track. For this reason, outfield walls are typically padded for extra safety. Wrigley Field's brick wall is covered only by ivy, which is not especially soft. However, there are pads on the walls of the tight left and right field corners in foul ground.
Warning-track power is a derogatory term for a batter who seems to have just enough power to hit the ball to the warning track for an out, but not enough to hit a home run. The term more generally refers to someone or something that is almost but not quite good enough for something.
Outfield wall[edit]
The Green Monster in 2006, showing the manual scoreboard and Green Monster seating, and more recent additions, including charity advertisements along the top, billboards above the Green Monster seating, and the American League East standings.
Pitchers warming up in the bullpen
The outfield wall or outfield fence is the wall or fence that marks the outer boundary of the outfield. A ball passing over the wall is dead; if it passes over the wall in fair territory while in flight, it is a home run. The official rules do not specify the shape, height, or composition of the wall, or a specific mandatory distance from home plate (though Major League Baseball mandates a minimum distance of 250 feet (76 m) and recommends a minimum distance of 320 feet (98 m) at the foul poles and 400 feet (120 m) at center field). As a result, baseball fields can vary greatly along those lines. The wall has numbers affixed or painted on it that denote the distance in feet from that point on the wall to home plate. In most modern major league ballparks, the wall is made of some hard material (e.g., concrete, plywood, sheet metal) with padding on the field side to protect players who may collide with the wall at high speed while trying to make a play. Chain link fencing may also be incorporated into the wall in areas where the wall needs to be transparent, e.g., an outfield bullpen, a spectator area behind the wall, or to protect a scoreboard incorporated into the wall. Many ballparks feature a yellow line denoting the top of the wall to aid umpires in judging whether the ball passed over the wall or if the ball is fair or foul.
Bullpen[edit]
Main article: Bullpen
The bullpen (sometimes referred to as simply "the pen") is the area where pitchers warm up before entering a game. Depending on the ballpark, it may be situated in foul territory along the baselines or just beyond the outfield fence. Relief pitchers usually wait in the bullpen when they have yet to play in a game, rather than in the dugout with the rest of the team. The starting pitcher also makes his final pregame warmups in the bullpen. Managers can call coaches in the bullpen on an in-house telephone from the dugout to tell a certain pitcher to begin his warmup tosses. "Bullpen" is also used metonymically to describe a team's collection of relief pitchers.
On-deck circles[edit]
Main article: On-deck
There are two on-deck circles in the field, one for each team, positioned in foul ground between home plate and the respective teams' benches. They are technically known as next-batter's boxes. The on-deck circle is where the next scheduled batter, or "on-deck" batter, warms up while waiting for the current batter to finish his turn. The on-deck circle is either an area composed of bare dirt; a plain circle painted onto artificial turf; or often, especially at the professional level, a mat made from artificial material, with the team or league logo painted onto it.
Coach's boxes[edit]
The coach's boxes, located behind first and third base, are where the first and third base coaches are supposed to stand, although coaches often stand outside the box. This is permissible as long as the coach does not interfere with play and the opposing team does not object (in which case the umpire shall ensure that all coaches on both teams must abide by the boundaries of the coach's boxes). The coach's boxes are marked with chalk or paint. In the early days of baseball, the term "coacher's box" was used, as "coach" was taken to be a verb. As the term "coach" evolved into a noun, the name of the box also changed.
History[edit]
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The basic layout of the diamond has been little changed since the original Knickerbocker Rules of the 1840s. The distance between bases was already established as 90 feet (27.5 m), which it remains to this day. Through trial and error, 90 feet (27.5 m) had been settled upon as the optimal distance. 100 feet (30.5 m) would have given too much advantage to the defense, and 80 feet too much to the offense.
The original Knickerbocker Rules did not specify the pitching distance explicitly. By the time major league baseball began in the 1870s, the pitcher was compelled to pitch from within a "box" whose front edge was 45 feet (13.7 m)from the "point" of home plate. Although he had to release the ball before crossing the line, as with bowlers in cricket, he also had to start his delivery from within the box; he could not run in from the field as bowlers do. Furthermore, the pitcher had to throw underhand. By the 1880s, pitchers had mastered the underhand delivery—in fact, in 1880, there were two perfect games within a week of each other.[10]
In an attempt to "increase the batting", the front edge of the pitcher's box was moved back 5 feet (1.5 m) in 1881, to 50 feet (15 m) from home plate. The size of the box was altered over the following few years. Pitchers were allowed to throw overhand starting in 1884, and that tilted the balance of power again. In 1887, the box was set at 4 feet (1.2 m) wide and 5.5 feet (1.7 m) deep, with the front edge still 50 feet (15 m) from the plate. However, the pitcher was compelled to deliver the ball with his back foot at the 55.5 foot (16.9 m) line of the box, thus somewhat restricting his ability to "power" the ball with his overhand delivery.[11]:96
In 1893, the box was replaced by the pitcher's plate, although "the box" is still used today as a slang term for the pitcher's location on the field. Exactly 5 feet (1.5 m) was added to the point the pitcher had to toe, again "to increase the batting" (and hopefully to increase attendance, as fan interest had flagged somewhat), resulting in the seemingly peculiar pitching distance of 60.5 feet (18.4 m).[11]:230
Many sources suggest that the pitching distance evolved from 45 to 50 to 60.5 feet. However, the first two were the "release point" and the third is the "pushoff point", so the 1893 increase was not quite as dramatic as is often implied; that is, the 1893 rule change added only 5 feet to the release point, not 10.5 feet (3.2 m).
Originally the pitcher threw from flat ground, but over time the raised mound was developed, somewhat returning the advantage to the pitchers. Before the mid-20th century, it was common for baseball fields to include a dirt pathway between the pitcher's mound and home plate. This feature is sometimes known as the "keyhole" due to the shape that it makes together with the mound. The keyhole was once as wide as the pitcher's box and resembled a cricket pitch. Sometimes this path extended through the batting area and all the way to the backstop. Once the rounded pitcher's mound was developed, the path became more ornamental than practical, and was gradually thinned before being largely abandoned by the 1950s. In recent years some ballparks, such as Comerica Park and Chase Field in the major leagues, have revived the feature for nostalgic reasons.
Maintenance[edit]
See: Turf management, Sports turf, Groundskeeping#Groundskeeping equipment, Equipment manager, and Sand-based athletic fields
Honors and awards[edit]
See also: ABCA/Turface Athletics Field Maintenance Awards
The Sports Turf Managers Association (STMA) presents various awards each year. Starting in 2001, its Sports Turf Manager of the Year Awards have been presented annually in the Triple-A, Double-A, Class A, and Short-Season/Rookie divisions of Minor League Baseball and are chosen from the 16 league winners.[12] STMA also presents the Baseball Field of the Year Award, which includes Schools and Parks, College/University and Professional categories.[13]
See also[edit]
Official Rules of Major League Baseball
The Baseball Encyclopedia, published by Macmillan Publishers
References[edit]
^ Jump up to: a b "Official Rules: 1.00 Objectives of the Game". Major League Baseball. Retrieved 26 October 2014.
Jump up ^ "DIAGRAM NO 2". Official Rules of Major League Baseball, 2013 Edition (PDF). Triumph Books. p. 5. ISBN 978-1-60078-797-3.
Jump up ^ James, B. New Historical Baseball Abstract.
Jump up ^ Weisstein, Eric W. "Home Plate". MathWorld.
Jump up ^ "Field Dimensions". MLB.com. Retrieved March 5, 2018.
Jump up ^ "Official Rules". Major League Baseball. Retrieved 14 October 2015.
Jump up ^ Mahony, Phillip (2014). Baseball Explained. McFarland Books.
Jump up ^ "Toronto Blue Jays' new dirt infield under construction at Rogers Centre". CBC.ca. February 10, 2016.
Jump up ^ "Major League Baseball Ballpark Grass and Turf". www.baseballpilgrimages.com. Retrieved 2018-03-06.
Jump up ^ "Lee Richmond Perfect Game Box Score by Baseball Almanac". baseball-almanac.com. Retrieved 14 October 2015.
^ Jump up to: a b Lansch, Jerry. Glory Fades Away.
Jump up ^ "Turf award winners named: Stevenson, Parrott, Tanis, Hall are classification winners". Florida State League official website. Minor League Baseball. November 21, 2011. Retrieved 2012-01-01. [T]he STMA [Sports Turf Managers Association] began recognizing sports turf managers in 2001.
Jump up ^ "Baseball: Hawks Field at Haymarket Park". Nebraska Huskers. Retrieved 2012-01-01. Hawks Field at Haymarket Park ... won the Baseball Field of the Year Award in the College/University division by the Sports Turf Managers Association (STMA) for the second time.
External links[edit]
Wikimedia Commons has media related to Baseball fields.
Baseball portal
Baseball field Almanac
PONY Baseball
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Blood Brother (upcoming film)
From Wikipedia, the free encyclopedia
Jump to: navigation, search
Blood Brother
Directed by
John Pogue
Produced by
Michael Luisi
Written by
Michael Finch
Karl Gajdusek
Charles Murray
Starring
Trey Songz
Music by
Junkie XL
Cinematography
Matthew Irving
Edited by
Lester Wilson
Production
company
WWE Studios
Codeblack Films
Distributed by
Codeblack Films
Country
United States
Language
English
Blood Brother is an upcoming American action thriller film directed by John Pogue and written by Michael Finch, Karl Gajdusek, and Charles Murray. The film stars Trey Songz.
Contents
[hide]
1 Plot
2 Cast
3 Production
4 References
5 External links
Plot[edit]
Blood Brother takes place on the mean streets of a city in decay, where a recently released convict begins to take murderous revenge against his childhood friends, whom he believes let him take the fall for a crime they collectively committed. As the bodies start piling up, one of the friends Sonny (Trey Songz), now a cop, will stop at nothing to put an end to the murderous rampage and to right the many wrongs of their tragically violent past. It also includes China Anne McClain, Jack Kesy, Hassan Johnson, Fetty Wap, and Jessica Re' Williams as Donna.
Cast[edit]
Trey Songz[1] as Sonny
R-Truth[2]
Fetty Wap as Emilio
Hassan Johnson as Joe
China Anne McClain as Darcy
Production[edit]
Principal photography on the film began on January 13, 2016 in New Orleans.[3][4] WWE Studios would be co-producing and co-financing the film under a deal with Lionsgate's CodeBlack Entertainment, with Michael Luisi producing for WWE.[1]
References[edit]
^ Jump up to: a b Hipes, Patrick (December 3, 2015). "WWE Studios & Lionsgate's Codeblack Films Unveil Co-Production Deal; First Pic Is Trey Songz-Starring 'Brother's Blood'". Deadline. Retrieved January 25, 2016.
Jump up ^ R-Truth To Appear In Upcoming WWE Studios Movie
Jump up ^ "WWE's 'Brother's Blood' Movie is Now Hiring Stand-ins in New Orleans". Project Casting. Retrieved January 25, 2016.
Jump up ^ "On the Set for 1/22/16: Michael Fassbender Starts Shooting Universal's 'The Snowman', Antonio Banderas Wraps on 'Security'". www.ssninsider.com. January 22, 2016. Retrieved January 25, 2016.
External links[edit]
Blood Brother on IMDb
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Real-time polymerase chain reaction
From Wikipedia, the free encyclopedia
Jump to: navigation, search
For reverse transcription polymerase chain reaction (RT-PCR), see reverse transcription polymerase chain reaction.
SYBR Green fluorescence chart produced in real-time PCR.
Melting curve produced at the end of real-time PCR.
A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR), and semi-quantitatively, i.e. above/below a certain amount of DNA molecules (semi quantitative real-time PCR).
Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.
The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR.[1] The acronym "RT-PCR" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.[2]
Contents
[hide]
1 Background
2 Basic principles
3 Chemical classification
3.1 Non-specific detection: Real-time PCR with double-stranded DNA-binding dyes as reporters
3.2 Specific detection: fluorescent reporter probe method
4 Fusion temperature analysis
4.1 Modeling
5 Applications
5.1 Quantification of gene expression
5.2 Diagnostic uses
5.3 Microbiological uses
5.4 Uses in research
5.5 Detection of phytopathogens
5.6 Detection of genetically modified organisms
5.7 Clinical quantification and genotyping
5.8 Environmental remediation applications
6 References
7 Bibliography
Background[edit]
Real time PCR uses fluorophores in order to detect levels of gene expression.
Cells in all organisms regulate gene expression by turnover of gene transcripts (single stranded RNA): The amount of an expressed gene in a cell can be measured by the number of copies of an RNA transcript of that gene present in a sample. In order to robustly detect and quantify gene expression from small amounts of RNA, amplification of the gene transcript is necessary. The polymerase chain reaction (PCR) is a common method for amplifying DNA; for RNA-based PCR the RNA sample is first reverse-transcribed to complementary DNA (cDNA) with reverse transcriptase.
In order to amplify small amounts of DNA, the same methodology is used as in conventional PCR using a DNA template, at least one pair of specific primers, deoxyribonucleotides, a suitable buffer solution and a thermo-stable DNA polymerase. A substance marked with a fluorophore is added to this mixture in a thermal cycler that contains sensors for measuring the fluorescence of the fluorophore after it has been excited at the required wavelength allowing the generation rate to be measured for one or more specific products. This allows the rate of generation of the amplified product to be measured at each PCR cycle. The data thus generated can be analysed by computer software to calculate relative gene expression (or mRNA copy number) in several samples. Quantitative PCR can also be applied to the detection and quantification of DNA in samples to determine the presence and abundance of a particular DNA sequence in these samples.[3] This measurement is made after each amplification cycle, and this is the reason why this method is called real time PCR (that is, immediate or simultaneous PCR). In the case of RNA quantitation, the template is complementary DNA (cDNA), which is obtained by reverse transcription of ribonucleic acid (RNA). In this instance the technique used is quantitative RT-PCR or Q-RT-PCR.
Quantitative PCR and DNA microarray are modern methodologies for studying gene expression. Older methods were used to measure mRNA abundance: Differential display, RNase protection assay and Northern blot. Northern blotting is often used to estimate the expression level of a gene by visualizing the abundance of its mRNA transcript in a sample. In this method, purified RNA is separated by agarose gel electrophoresis, transferred to a solid matrix (such as a nylon membrane), and probed with a specific DNA or RNA probe that is complementary to the gene of interest. Although this technique is still used to assess gene expression, it requires relatively large amounts of RNA and provides only qualitative or semi quantitative information of mRNA levels.[4] Estimation errors arising from variations in the quantification method can be the result of DNA integrity, enzyme efficiency and many other factors. For this reason a number of standardization systems (often called normalization methods) have been developed. Some have been developed for quantifying total gene expression, but the most common are aimed at quantifying the specific gene being studied in relation to another gene called a normalizing gene, which is selected for its almost constant level of expression. These genes are often selected from housekeeping genes as their functions related to basic cellular survival normally imply constitutive gene expression.[5][6] This enables researchers to report a ratio for the expression of the genes of interest divided by the expression of the selected normalizer, thereby allowing comparison of the former without actually knowing its absolute level of expression.
The most commonly used normalizing genes are those that code for the following molecules: tubulin, glyceraldehyde-3-phosphate dehydrogenase, albumin, cyclophilin, and ribosomal RNAs.[4]
Basic principles[edit]
Main article: Polymerase chain reaction
Real-time PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore. The thermal cycler is also able to rapidly heat and chill samples, thereby taking advantage of the physicochemical properties of the nucleic acids and DNA polymerase.
The PCR process generally consists of a series of temperature changes that are repeated 25 – 50 times. These cycles normally consist of three stages: the first, at around 95 °C, allows the separation of the nucleic acid's double chain; the second, at a temperature of around 50-60 °C, allows the binding of the primers with the DNA template;[7] the third, at between 68 - 72 °C, facilitates the polymerization carried out by the DNA polymerase. Due to the small size of the fragments the last step is usually omitted in this type of PCR as the enzyme is able to increase their number during the change between the alignment stage and the denaturing stage. In addition, in four step PCR the fluorescence is measured during short temperature phase lasting only a few seconds in each cycle, with a temperature of, for example, 80 °C, in order to reduce the signal caused by the presence of primer dimers when a non-specific dye is used.[8] The temperatures and the timings used for each cycle depend on a wide variety of parameters, such as: the enzyme used to synthesize the DNA, the concentration of divalent ions and deoxyribonucleotides (dNTPs) in the reaction and the bonding temperature of the primers.[9]
Chemical classification[edit]
Real-time PCR technique can be classified by the chemistry used to detect the PCR product, specific or non-specific fluorochromes.
Non-specific detection: Real-time PCR with double-stranded DNA-binding dyes as reporters[edit]
A DNA-binding dye binds to all double-stranded (ds) DNA in PCR, causing fluorescence of the dye. An increase in DNA product during PCR therefore leads to an increase in fluorescence intensity measured at each cycle. However, dsDNA dyes such as SYBR Green will bind to all dsDNA PCR products, including nonspecific PCR products (such as Primer dimer). This can potentially interfere with, or prevent, accurate monitoring of the intended target sequence.
In real-time PCR with dsDNA dyes the reaction is prepared as usual, with the addition of fluorescent dsDNA dye. Then the reaction is run in a real-time PCR instrument, and after each cycle, the intensity of fluorescence is measured with a detector; the dye only fluoresces when bound to the dsDNA (i.e., the PCR product). This method has the advantage of only needing a pair of primers to carry out the amplification, which keeps costs down; multiple target sequences can be monitored in a tube by using different types of dyes.
Specific detection: fluorescent reporter probe method[edit]
(1) In intact probes, reporter fluorescence is quenched. (2) Probes and the complementary DNA strand are hybridized and reporter fluorescence is still quenched. (3) During PCR, the probe is degraded by the Taq polymerase and the fluorescent reporter released.
Fluorescent reporter probes detect only the DNA containing the sequence complementary to the probe; therefore, use of the reporter probe significantly increases specificity, and enables performing the technique even in the presence of other dsDNA. Using different-coloured labels, fluorescent probes can be used in multiplex assays for monitoring several target sequences in the same tube. The specificity of fluorescent reporter probes also prevents interference of measurements caused by primer dimers, which are undesirable potential by-products in PCR. However, fluorescent reporter probes do not prevent the inhibitory effect of the primer dimers, which may depress accumulation of the desired products in the reaction.
The method relies on a DNA-based probe with a fluorescent reporter at one end and a quencher of fluorescence at the opposite end of the probe. The close proximity of the reporter to the quencher prevents detection of its fluorescence; breakdown of the probe by the 5' to 3' exonuclease activity of the Taq polymerase breaks the reporter-quencher proximity and thus allows unquenched emission of fluorescence, which can be detected after excitation with a laser. An increase in the product targeted by the reporter probe at each PCR cycle therefore causes a proportional increase in fluorescence due to the breakdown of the probe and release of the reporter.
The PCR is prepared as usual (see PCR), and the reporter probe is added.
As the reaction commences, during the annealing stage of the PCR both probe and primers anneal to the DNA target.
Polymerisation of a new DNA strand is initiated from the primers, and once the polymerase reaches the probe, its 5'-3'-exonuclease degrades the probe, physically separating the fluorescent reporter from the quencher, resulting in an increase in fluorescence.
Fluorescence is detected and measured in a real-time PCR machine, and its geometric increase corresponding to exponential increase of the product is used to determine the quantification cycle (Cq) in each reaction.
Fusion temperature analysis[edit]
Distinct fusion curves for a number of PCR products (showing distinct colours). Amplification reactions can be seen for a specific product (pink, blue) and others with a negative result (green, orange). The fusion peak indicated with an arrow shows the peak caused by primer dimers, which is different from the expected amplification product.[10]
Real-time PCR permits the identification of specific, amplified DNA fragments using analysis of their melting temperature (also called Tm value, from melting temperature). The method used is usually PCR with double-stranded DNA-binding dyes as reporters and the dye used is usually SYBR Green. The DNA melting temperature is specific to the amplified fragment. The results of this technique are obtained by comparing the dissociation curves of the analysed DNA samples.[11]
Unlike conventional PCR, this method avoids the previous use of electrophoresis techniques to demonstrate the results of all the samples. This is because, despite being a kinetic technique, quantitative PCR is usually evaluated at a distinct end point. The technique therefore usually provides more rapid results and / or uses fewer reactants than electrophoresis. If subsequent electrophoresis is required it is only necessary to test those samples that real time PCR has shown to be doubtful and / or to ratify the results for samples that have tested positive for a specific determinant.
Modeling[edit]
Unlike end point PCR (conventional PCR), real time PCR allows monitoring of the desired product at any point in the amplification process by measuring fluorescence (in real time frame, measurement is made of its level over a given threshold). A commonly employed method of DNA quantification by real-time PCR relies on plotting fluorescence against the number of cycles on a logarithmic scale. A threshold for detection of DNA-based fluorescence is set 3-5 times of the standard deviation of the signal noise above background. The number of cycles at which the fluorescence exceeds the threshold is called the threshold cycle (Ct) or, according to the MIQE guidelines, quantification cycle (Cq).[12]
During the exponential amplification phase, the quantity of the target DNA template (amplicon) doubles every cycle. For example, a DNA sample whose Cq precedes that of another sample by 3 cycles contained 23 = 8 times more template. However, the efficiency of amplification is often variable among primers and templates. Therefore, the efficiency of a primer-template combination is assessed in a titration experiment with serial dilutions of DNA template to create a standard curve of the change in (Cq) with each dilution. The slope of the linear regression is then used to determine the efficiency of amplification, which is 100% if a dilution of 1:2 results in a (Cq) difference of 1. The cycle threshold method makes several assumptions of reaction mechanism and has a reliance on data from low signal-to-noise regions of the amplification profile that can introduce substantial variance during the data analysis.[13]
To quantify gene expression, the (Cq) for an RNA or DNA from the gene of interest is subtracted from the (Cq) of RNA/DNA from a housekeeping gene in the same sample to normalize for variation in the amount and quality of RNA between different samples. This normalization procedure is commonly called the ΔCt-method[14] and permits comparison of expression of a gene of interest among different samples. However, for such comparison, expression of the normalizing reference gene needs to be very similar across all the samples. Choosing a reference gene fulfilling this criterion is therefore of high importance, and often challenging, because only very few genes show equal levels of expression across a range of different conditions or tissues.[15][16] Although cycle threshold analysis is integrated with many commercial software systems, there are more accurate and reliable methods of analysing amplification profile data that should be considered in cases where reproducibility is a concern.[13]
Mechanism-based qPCR quantification methods have also been suggested, and have the advantage that they do not require a standard curve for quantification. Methods such as MAK2[17] have been shown to have equal or better quantitative performance to standard curve methods. These mechanism-based methods use knowledge about the polymerase amplification process to generate estimates of the original sample concentration. An extension of this approach includes an accurate model of the entire PCR reaction profile, which allows for the use of high signal-to-noise data and the ability to validate data quality prior to analysis.[13]
According to research of Ruijter et al.[18] MAK2 assumes constant amplification efficiency during the PCR reaction. However, theoretical analysis of polymerase chain reaction, from which MAK2 was derived, has revealed that amplification efficiency is not constant throughout PCR. While MAK2 quantification provides reliable estimates of target DNA concentration in a sample under normal qPCR conditions, MAK2 does not reliably quantify target concentration for qPCR assays with competimeters.
Applications[edit]
There are numerous applications for quantitative polymerase chain reaction in the laboratory. It is commonly used for both diagnostic and basic research. Uses of the technique in industry include the quantification of microbial load in foods or on vegetable matter, the detection of GMOs (Genetically modified organisms) and the quantification and genotyping of human viral pathogens.
Quantification of gene expression[edit]
Quantifying gene expression by traditional DNA detection methods is unreliable. Detection of mRNA on a Northern blot or PCR products on a gel or Southern blot does not allow precise quantification.[19] For example, over the 20-40 cycles of a typical PCR, the amount of DNA product reaches a plateau that is not directly correlated with the amount of target DNA in the initial PCR.[citation needed]
Real-time PCR can be used to quantify nucleic acids by two common methods: relative quantification and absolute quantification.[20] Absolute quantification gives the exact number of target DNA molecules by comparison with DNA standards using a calibration curve. It is therefore essential that the PCR of the sample and the standard have the same amplification efficiency.[21] Relative quantification is based on internal reference genes to determine fold-differences in expression of the target gene. The quantification is expressed as the change in expression levels of mRNA interpreted as complementary DNA (cDNA, generated by reverse transcription of mRNA). Relative quantification is easier to carry out as it does not require a calibration curve as the amount of the studied gene is compared to the amount of a control reference gene.
As the units used to express the results of relative quantification are unimportant the results can be compared across a number of different RT-Q-PCR. The reason for using one or more housekeeping genes is to correct non-specific variation, such as the differences in the quantity and quality of RNA used, which can affect the efficiency of reverse transcription and therefore that of the whole PCR process. However, the most crucial aspect of the process is that the reference gene must be stable.[22]
The selection of these reference genes was traditionally carried out in molecular biology using qualitative or semi-quantitative studies such as the visual examination of RNA gels, Northern blot densitometry or semi-quantitative PCR (PCR mimics). Now, in the genome era, it is possible to carry out a more detailed estimate for many organisms using transcriptomic technologies.[23] However, research has shown that amplification of the majority of reference genes used in quantifying the expression of mRNA varies according to experimental conditions.[24][25][26] It is therefore necessary to carry out an initial statistically sound methodological study in order to select the most suitable reference gene.
A number of statistical algorithms have been developed that can detect which gene or genes are most suitable for use under given conditions. Those like geNORM or BestKeeper can compare pairs or geometric means for a matrix of different reference genes and tissues.[27][28]
Diagnostic uses[edit]
Diagnostic qualitative PCR is applied to rapidly detect nucleic acids that are diagnostic of, for example, infectious diseases, cancer and genetic abnormalities. The introduction of qualitative PCR assays to the clinical microbiology laboratory has significantly improved the diagnosis of infectious diseases,[29] and is deployed as a tool to detect newly emerging diseases, such as new strains of flu, in diagnostic tests.[30]
Microbiological uses[edit]
Quantitative PCR is also used by microbiologists working in the fields of food safety, food spoilage and fermentation and for the microbial risk assessment of water quality (drinking and recreational waters) and in public health protection.[31]
Uses in research[edit]
In research settings, quantitative PCR is mainly used to provide quantitative measurements of gene transcription. The technology may be used in determining how the genetic expression of a particular gene changes over time, such as in the response of tissue and cell cultures to an administration of a pharmacological agent, progression of cell differentiation, or in response to changes in environmental conditions. It is also used for the determination of zygosity of transgenic animals used in research.
Detection of phytopathogens[edit]
The agricultural industry is constantly striving to produce plant propagules or seedlings that are free of pathogens in order to prevent economic losses and safeguard health. Systems have been developed that allow detection of small amounts of the DNA of Phytophthora ramorum, an oomycete that kills Oaks and other species, mixed in with the DNA of the host plant. Discrimination between the DNA of the pathogen and the plant is based on the amplification of ITS sequences, spacers located in ribosomal RNA gene's coding area, which are characteristic for each taxon.[32] Field-based versions of this technique have also been developed for identifying the same pathogen.[33]
Detection of genetically modified organisms[edit]
qPCR using reverse transcription (RT-qPCR) can be used to detect GMOs given its sensitivity and dynamic range in detecting DNA. Alternatives such as DNA or protein analysis are usually less sensitive. Specific primers are used that amplify not the transgene but the promoter, terminator or even intermediate sequences used during the process of engineering the vector. As the process of creating a transgenic plant normally leads to the insertion of more than one copy of the transgene its quantity is also commonly assessed. This is often carried out by relative quantification using a control gene from the treated species that is only present as a single copy.[34][35]
Clinical quantification and genotyping[edit]
Viruses can be present in humans due to direct infection or co-infections which makes diagnosis difficult using classical techniques and can result in an incorrect prognosis and treatment. The use of qPCR allows both the quantification and genotyping (characterization of the strain, carried out using melting curves) of a virus such as the Hepatitis B virus.[36] The degree of infection, quantified as the copies of the viral genome per unit of the patient's tissue, is relevant in many cases; for example, the probability that the type 1 herpes simplex virus reactivates is related to the number of infected neurons in the ganglia.[37] This quantification is carried out either with reverse transcription or without it, as occurs if the virus becomes integrated in the human genome at any point in its cycle, such as happens in the case of HPV (human papillomavirus), where some of its variants are associated with the appearance of cervical cancer.[38]
Environmental remediation applications[edit]
qPCR is used to evaluate biodegradation potential or activity in contaminated groundwater. qPCR is used to detect and quantify bacteria such as Dehalococcoides mccartyi as well as vinyl chloride reductase functional genes essential to the biodegradation of chlorinated solvents including trichloroethene and tetrachloroethylene. qPCR is also commonly used to quantify functional genes involved in the biodegradation of benzene, toluene, ethylbenzene, and xylenes (BTEX) and evaluate monitored natural attenuation (MNA) or other remedial methods at impacted petroleum hydrocarbon sites. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR), which is based on the analysis of RNA rather than DNA, is used to quantify gene expression and identify biodegradation activity.
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